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STAT5-Luc/BaF3

CBPB0001

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I. Background
After activation by cytokines or growth factors, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.
 
II. Introduction
Expressed gene: STAT5
Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+10ng/ml IL-3+200ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
 
III. Representative Data

Figure 1.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). STAT5 Luciferase Reporter - BaF3 Cells (C18),the EC50 was 0.23ng/ml.

 

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