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SRE.L-Luc/HEK293

CBP74201

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I. Background
The SRE Reporter – HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter – HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.
 
II. Introduction
Host Cell: HEK293
Expressed gene: SRE.L-Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): SRE cell line, MAPK reporter, ERK reporter
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+100ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
III. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent
 
IV. Representative Data

Figure 1.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer).

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