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首頁(yè) /藥靶模型 /GPCR靶點(diǎn)細胞 /Class A(Rhodopsin) /C5aR1/CHO

C5aR1/CHO

CBP71374

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I. Background
C5AR1 (Complement C5a Receptor 1) is a Protein Coding gene. Diseases associated with C5AR1 include Hypersensitivity Reaction Type Iii Disease and Mast-Cell Sarcoma. Among its related pathways are Complement cascade and GPCR downstream signalling.
 
II. Introduction
Host Cell:

CHO

Stability: 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: F12K+10%FBS+5ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen immediately upon delivery
Application(s):

Functional assay for C5aR1

Transducer: Gi
 
Ⅲ. Description of Host Cell Line
Organism: Hamster
Tissue: Ovary
Morphology: Epithelial
Growth Properties: Adherent
 
Ⅳ. Representative Data

Figure 1. Recombinant C5aR1/CHO constitutively expressing C5aR1.

Figure 2. HTRF cAMP Assay With C5aR1 CHO Cells (C7).


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